RESUMO
The bacterium, Inquilinus limosus, with its remarkable antimicrobial multiresistant profile, has increasingly been isolated in cystic fibrosis patients. We report draft genome sequence of a strain MP06, which is of considerable interest in elucidating the associated mechanisms of antibiotic resistance in this bacterium and for an insight about its persistence in airways of these patients.
Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla , Genoma Bacteriano , Infecções por Bactérias Gram-Negativas/microbiologia , Rhodospirillaceae/genética , Sequência de Bases , Humanos , Dados de Sequência Molecular , Rhodospirillaceae/efeitos dos fármacosRESUMO
The bacterium, Inquilinus limosus, with its remarkable antimicrobial multiresistant profile, has increasingly been isolated in cystic fibrosis patients. We report draft genome sequence of a strain MP06, which is of considerable interest in elucidating the associated mechanisms of antibiotic resistance in this bacterium and for an insight about its persistence in airways of these patients.
Assuntos
Antibacterianos/efeitos dos fármacos , Antibacterianos/genética , Antibacterianos/microbiologia , Antibacterianos/farmacologia , Sequência de Bases/efeitos dos fármacos , Sequência de Bases/genética , Sequência de Bases/microbiologia , Sequência de Bases/farmacologia , Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos , Farmacorresistência Bacteriana Múltipla/genética , Farmacorresistência Bacteriana Múltipla/microbiologia , Farmacorresistência Bacteriana Múltipla/farmacologia , Genoma Bacteriano/efeitos dos fármacos , Genoma Bacteriano/genética , Genoma Bacteriano/microbiologia , Genoma Bacteriano/farmacologia , Infecções por Bactérias Gram-Negativas/efeitos dos fármacos , Infecções por Bactérias Gram-Negativas/genética , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/farmacologia , Humanos/efeitos dos fármacos , Humanos/genética , Humanos/microbiologia , Humanos/farmacologia , Dados de Sequência Molecular/efeitos dos fármacos , Dados de Sequência Molecular/genética , Dados de Sequência Molecular/microbiologia , Dados de Sequência Molecular/farmacologia , Rhodospirillaceae/efeitos dos fármacos , Rhodospirillaceae/genética , Rhodospirillaceae/microbiologia , Rhodospirillaceae/farmacologiaAssuntos
Alphaproteobacteria/genética , Proteínas de Bactérias/genética , Cromossomos/genética , Resistência beta-Lactâmica/genética , beta-Lactamases/genética , Alphaproteobacteria/enzimologia , Alphaproteobacteria/isolamento & purificação , Proteínas de Bactérias/isolamento & purificação , Humanos , Testes de Sensibilidade Microbiana/métodos , beta-Lactamases/isolamento & purificaçãoRESUMO
Two types of hydrophilic networks with conjugated beta-cyclodextrin (ß-CD) were developed with the aim of engineering useful platforms for the localized release of an antimicrobial 5,6-dimethoxy-1-indanone N4-allyl thiosemicarbazone (TSC) in the eye and its potential application in ophthalmic diseases. Poly(2-hydroxyethyl methacrylate) soft contact lenses (SCLs) displaying ß-CD, namely pHEMA-co-ß-CD, and super-hydrophilic hydrogels (SHHs) of directly cross-linked hydroxypropyl-ß-CD were synthesized and characterized regarding their structure (ATR/FT-IR), drug loading capacity, swelling and in vitro release in artificial lacrimal fluid. Incorporation of TSC to the networks was carried out both during polymerization (DP method) and after synthesis (PP method). The first method led to similar drug loads in all the hydrogels, with minor drug loss during the washing steps to remove unreacted monomers, while the second method evidenced the influence of structural parameters on the loading efficiency (proportion of CD units, mesh size, swelling degree). Both systems provided a controlled TSC release for at least two weeks, TSC concentrations (up to 4000µg/g dry hydrogel) being within an optimal therapeutic window for the antimicrobial ocular treatment. Microbiological tests against P. aeruginosa and S. aureus confirmed the ability of TSC-loaded pHEMA-co-ß-CD network to inhibit bacterial growth.
